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The soy plant was modified with the insertion of the Cry1Ac protein
which provides protection from feeding damage caused by targeted
lepidopteran pests, such as primary target pests velvetbean
caterpillar (Anticarcia gemmatalis), soybean looper (Pseudoplusia
includens), soybean anxil borer (Epinotia aporema), and sunflower
looper (Rachiplusia nu).
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
- Agrobacterium-mediated DNA transfer
MON 87701 was developed through transformation of soybean meristem
tissues using the binary transformation plasmid PV-GMIR9 which
contains two T-DNAs delineated by left and right border sequences
which facilitate transformation. The first T-DNA, designated as
T-DNA I, contains the cry1Ac expression cassette. The second T-DNA,
designated as T-DNA II, contains the cp4 epsps expression cassette.
The Cry1Ac coding sequence was modified for plant optimised codons
and resulted in a single amino acid change at L766S with four
additional codons at the N-terminus from the CTP2 genetic
Molecular characterization of MON 87701 by Southern blot analyses
demonstrated that the DNA inserted into the soybean genome is
present at a single locus and contains one functional copy of the
cry1Ac expression cassette. No TDNA II (cp4 epsps gene expression
cassette) genetic elements or backbone sequences from the
transformation plasmid were detected in MON 87701. In addition, no
partial genetic elements, linked or unlinked to the inserted
expression cassette were detected.
T-DNA II expression Cassette: FMV 35S promoter >> EPSPS
Leader >> CTP2 >> EPSPS gene >> rbcS-E9 gene
- Resistance to diseases and pests
- Lepidoptera (butterflies and moths)
Utilizing a vector with two T-DNAs is the basis for an effective
approach to generate marker-free plants. It allows for the TDNA
with the traits of interest (T-DNA I) and the T-DNA encoding the
selectable marker (T-DNA II) to be inserted into two independent
loci within the genome of the plant. Following selection of the
transformants, the inserted T-DNA encoding the selectable marker
can be segregated from progeny through subsequent traditional
breeding and genetic selection processes, while the inserted T-DNA
containing the trait(s) of interest is maintained resulting in an
LMO that marker-free and contains only the cry1Ac expression