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Modified Organism
MON-89Ø34-3 x MON-ØØ6Ø3-6 - Genuity® VT Double Pro™ Maize
Record information and status
Record ID
46305
Status
Published
Date of creation
2008-08-07 09:40 UTC (manoela.miranda@cbd.int)
Date of last update
2020-04-06 13:19 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-04-06 13:19 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Genuity® VT Double Pro™ Maize
Transformation event
MON89034 x NK603
Unique identifier
MON-89Ø34-3 x MON-ØØ6Ø3-6
Developer(s)
Monsanto Europe S.A.
Avenue de Tervuren 270-272
Brussels
Belgium, B-1150
Description
The stacked maize line was obtained through the traditional cross-breeding of the parental lines MON-89Ø34-3 and MON-ØØ6Ø3-6. The modified maize expresses Bacillus thuringiensis cry1A.105 and cry2Ab2, which confer resistance to Lepidoptera pests. The line also contains two Agrobacterium tumefaciens epsps gene cassettes for tolerance to glyphosate.The bacterial epsps gene contains a sequence variation, which allows for tolerance to the glyphosate herbicide.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
MON-89Ø34-3 - YieldGard™ VT Pro™
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
MON-ØØ6Ø3-6 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMGT32 and  PV-ZMIR245
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.30 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cry1A.105
3.53 Kb
 
 
Heat shock protein 17.3 terminator
0.21 Kb
 
 
FMV 35S promoter
0.56 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Transit peptide and first intron of Rubisco SSU
0.40 Kb
 
 
Cry2Ab2
1.91 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Rice actin 1 gene promoter
0.80 Kb
 
 
Rice actin 1, intron
0.60 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
CaMV Enhanced 35S promoter
0.60 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON89034 vector PV-ZMIR245:
Maize line MON89034 expresses two Bt-toxins encoded by the Bacillus thuringiensis genes cry1A.105 and cry2Ab2.

Transcription of cry1A.105 begins are the Cauliflower Mosaic Virus (CaMV) 35S promoter and finishes at the wheat (Triticum aestivum) wheat heat shock protein 17.3 terminator. The transcript initially includes (5' to 3'): wheat 5' untranslated leader from the chlorophyll a/b-binding protein, Oryza sativa (rice) actin 1 intron and cry1A.105. The wheat 5' untranslated leader sequence and the rice intron enhance expression of cry1A.105.

Transcription of cry2Ab2 commences from the Figwort Mosaic Virus (FMV) 35S promoter and terminates at the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The transcript initially includes (5' to 3'): maize heat shock protein 70 (Hsp70) intron, maize transit peptide and first intron from the small subunit of Rubsico and cry2Ab32. The Hsp70 regulates and enhances gene expression, while the transit peptide targets cr2Ab2 to the chloroplast.

Note:
- The viral promoters are expected to be constitutively active and promote high levels of transcription.
- The coding sequence of cry2Ab2 was codon-optimized for expression within plant systems.
- A second T-DNA insertion (containing CaMV 35S promoter, Escherichia coli neomycin phosphotransferase and A. tumefaciens nos terminator) was initially inserted into the genome for kanamycin selection during transformation. However, once transformants were regenerated, the selectable marker was bred out of the parental line using convention breeding techniques.
- Southern blot analyses indicated a single copy of the cry1A.105 and the cry2Ab2 cassettes. No backbone plasmid DNA or nptII sequences were detected. PCR and DNA sequence analyses provided the complete DNA sequence of the insert and confirmed the organization of the elements within the insert. Furthermore, sequence analysis indicated that MON 89034 no longer has the duplicated enhancer elements compared to the original e35S promoter in PV-ZMIR245, possibly due to a recombination event that resulted in its deletion.


DNA insert from NK603, vector PV-ZMGT32:
The plant expression plasmid vector, PV-ZMGT32 contains two adjacent plant gene expression cassettes each containing a single copy of the Agrobacterium tumefaciens strain CP4 5-enolpyruvylshikimate-3-phosphate synthase (epsps). In the first expression cassette (5' end), the epsps gene is under transcriptional control of an Oryza sativa (rice) Actin 1 promoter and the A. tumefaciens nopaline synthase (nos) terminator. During transcription, a rice Actin 1 intron and an Arabidopsis thaliana chloroplast transit peptide 2 are included upstream (5') of the epsps coding sequence. The rice intron enhances EPSPS expression and the transit peptide targets EPSPS to the chloroplasts of the plant cells. The second epsps cassette is under control of the Cauliflower Mosaic Virus 35S enhanced promoter and the nos terminator. Similarly, transcription additionally includes a maize heat shock protein 70 intron and an A. thaliana chloroplast transit peptide 2. The heat shock protein intron also enhances expression of epsps.

Note:
The parental line (NK603) has one insertion site containing both epsps gene cassettes. No vector backbone (neomycin phosphotransferase and origin of replication) sequences were detected.


For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Additional Information
Other relevant website address or attached documents

Records referencing this document (105)
IDDescription
105record(s) found
Country's Decision or any other Communication42 records
Information Resource1 record
Modified Organism1 record
Organization10 records
Risk Assessment51 records