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MON-88Ø17-3 x MON-ØØ81Ø-6
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
|Phone:||+ 1 314 694-1000|
|Fax:||+1 314 694-3080|
A stacked insect-resistant maize derived from conventional
cross-breeding of MON-88Ø17-3 and MON-ØØ81Ø-6. Resistance to
insect attack is conferred through expression of a truncated form
of the cry1Ab gene and cry3Bb1 gene from Bacillus thuringiensis
subsp. kurstaki HD-1 and tolerance to glyphosate-herbicides is
produced through introduction of the epsps gene from Agrobacterium
tumefaciens which confers tolerance to the herbicide Roundup, with
the active ingredient glyphosate.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
MON-88Ø17-3 - YieldGard™ VT™ Rootworm/RR2™ Maize
Resistance to diseases and pests - Insects - Coleoptera (beetles)
Resistance to herbicides - Glyphosate Show detection method(s)
PV-ZMIR39, PV-ZMBK07 and PV-ZMGT10
CaMV Enhanced 35S promoter
Rice actin 1 gene promoter
Chloroplast transit peptide 2
5-enolpyruvylshikimate-3-phosphate synthase gene
Nopaline Synthase Gene Terminator
CaMV Enhanced 35S promoter
5' untranslated leader from chlorophyll a/b-binding protein
Heat shock protein 17.3 terminator
DNA insert from MON810, vectors PV-ZMBK07 and
MON810 contains a truncated portion of a synthetic form of the
cry1Ab gene from Bacillus thuringiensis subsp. kurstaki. Two
constructs PV-ZMBK07 and PV-ZMGT10 have been used for
transformation, but molecular analyses showed that MON810 does not
contain any element from PV-ZMGT10 construct and only the elements
from construct PV-ZMBK07 have been integrated into its genome.
MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70
and cry1Ab. The terminator of the nopaline synthase (nos) gene was
lost due to a truncation at the 3' end of the gene cassette during
genome integration and is, therefore, not present in MON810.
DNA insert from MON88017 vector PV-ZMIR39
MON88017 expresses the cry3Bb1 gene encoding a Coleopteran-specific
insecticidal protein to control infestation with corn root worm,
and the cp4 epsps gene.
For additional information on this LMO, please refer to the records
of the parental LMOs.
- Resistance to diseases and pests
- Coleoptera (beetles)
- Lepidoptera (butterflies and moths)
- European corn borer (Ostrinia nubilalis)
- Resistance to herbicides
MON88017 x MON810 is resistant to insect attack and tolerant to use
of the herbicide glyphosate. The cry3Bb1 gene encodes a
Coleopteran-specific insecticidal protein from Bacillus
thuringiensis (subsp. kumamotoensis) in order to control
infestation with corn root worm, the cry1Ab gene produces the
insect control protein Cry1Ab, a delta-endotoxin which is
insecticidal to lepidopteran insects, and the cp4 epsps gene from
the soil bacterium Agrobacterium ssp. strain CP4 provides tolerance
to the herbicide glyphosate.
The cry3Bb1 gene encodes the insect control protein Cry3Bb1, a
delta-endotoxin, The toxin protects the plant from the Coleopteran
insects western corn rootworm (Diabrotica virgifera), northern corn
rootworm (D. barberi), and Mexican corn rootworm (D. virgifera
zeae). The cry1Ab gene produces the insect control protein
Cry1Ab and protects the plant against lepidopteran insects such as
the european corn borer (ECB).
Cry proteins act by selectively binding to specific sites localized
on the lining of the midgut of susceptible insect species.
Following binding, pores are formed that disrupt midgut ion flow,
causing gut paralysis and eventual death due to bacterial sepsis.
Cry3Bb1 is lethal only when eaten by Coleopteran species, including
corn root worm, and its specificity of action is directly
attributable to the presence of specific binding sites in the
target insects. Cry1Ab is insecticidal only when eaten by the
larvae of lepidopteran insects (moths and butterflies). There are
no binding sites for the delta-endotoxins of B. thuringiensis on
the surface of mammalian intestinal cells, therefore, livestock
animals and humans are not susceptible to these proteins.
The cp4 epsps gene encodes for a form of the plant enzyme
5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that is highly
tolerant to inhibition by glyphosate.
This line is a product of traditional plant breeding, and therefore
is not automatically subject to regulation in all jurisdictions as
are transgenic plants resulting from recombinant DNA technologies.
Certain jurisdictions may request notification in advance of the
release of a stacked hybrid, or may request information to conduct
an environmental and food safety assessment.