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Record details
Modified Organism
Cassava mosaic disease resistant cassava
LMO Information
Decisions on the LMO
Risk Assessments
Record information and status
Record ID
115622
Status
Published
Date of creation
2020-06-26 14:06 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-06-26 14:06 UTC (austein.mcloughlin@cbd.int)
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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the
LMO quick-links
page.
LMO name
Cassava mosaic disease resistant cassava
Transformation event
pCRNAi-dsAC1dsAV1_tripleintron
Developer(s)
Record #115618
Prof. Hassan Karakacha Were
Dean, School of Agriculture, Veterinary Science and Technology
Masinde Muliro University of Science and Technology (MMUST)
Kakamega
Kenya
Phone:
(+254) (724) (972207)
Email:
hwere@mmust.ac.ke
Description
The cassava was modified for resistance to Cassava mosaic disease (CMD) by introducing an RNA interference cassette that targets
African cassava mosaic virus
(ACMV) replication associated disease AC1 and capsid protein (AV1). The production of hairpin RNA by the host cells trigger an RNAi response that is expected to target viral transcripts and prevent viral replication and thus further infection. Due to conservation between AC1 and AV1 sequences in ACMV and
East african cassava mosaic virus
, the modified cassava is expected to resistant to both viruses, which can cause CMD. A selectable marker,
Escherichia coli
hygromycin B phosphotransferase, was additionally included for hygromycin selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Record #112539
Manihot esculenta - Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES
Related LMOs
Record #115625
Cassava mosaic disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Viruses Selectable marker genes and reporter genes
Record #115621
Cassava mosaic disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Viruses Selectable marker genes and reporter genes
Record #115623
Cassava mosaic disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes
Record #115624
Cassava brown streak disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pCAMBIA1300
Techniques used for the modification
Agrobacterium-mediated DNA transfer
Genetic elements construct
CaMV 35S promoter
#100287
0.00 Kb
Replication Associated Gene
#114697
0.15 Kb
M27939 Intron Sequence
#112601
0.20 Kb
Replication Associated Gene
#114697
0.15 Kb
M27939 Intron Sequence
#112601
0.20 Kb
Capsid protein
#115619
0.16 Kb
M27939 Intron Sequence
#112601
0.20 Kb
Capsid protein
#115619
0.16 Kb
Nopaline Synthase Gene Terminator
#100269
0.00 Kb
CaMV 35S promoter
#100287
0.00 Kb
Hygromycin B phosphotransferase gene
#14991
0.00 Kb
Nopaline Synthase Gene Terminator
#100269
0.00 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
RNA interference cassette
The modified cassava contains an RNA interference (RNAi) cassette designed to target
African cassava mosaic virus
replication associated gene (AC1) and capsid protein (AV1). Transcription is initiated from the
Cauliflower mosaic virus
(CaMV) 35S promoter and terminates at the CaMV 35S terminator. The transcript contains two segments (sense and antisense) of AC1 separated by a plant synthetic intron. Following the AC1 portion, the transcript contains another synthetic intron and then AV1 antisense and sense sequences separated another synthetic intron. Post-transcription, the introns between the sense and antisense sequences function as loops, allowing the sense and antisense segments of AC1 and AV1 to base pair, forming hpRNA. Two hpRNA structures separated by a synthetic intron are expected to be formed: one corresponding to the AC1 segment and one corresponding to the AV1 segment. The hpRNA acts as double stranded RNA (dsRNA), which triggers an RNAi response and the host cell machinery will target
AC1
and
AV1
viral transcripts for degradation.
Note:
- The segment of AC1 corresponds to position 1690 to 1844 of the ACMV DNA 1 (DNA A) (GenBank accession AJ427910).
- The segment of AV1 corresponds to position 492 to 647 of the ACMV DNA 1 (DNA A) (GenBank accession AJ427910).
- The source of the AC1 and AV1 sequences is ACMV strain Nigeria-Ogo.
- Due to the RNAi response, no protein translation is expected to occur from the transcript produced from the RNAi cassette.
Selectable marker
Transcription of
Escherichia coli
hygromycin B phosphotransferase is under transcriptional control of the CaMV 35S promoter and
Agrobacterium tumefaciens
nopaline synthase terminator.
LMO characteristics
Modified traits
Resistance to antibiotics
Hygromycin
Resistance to diseases and pests
Viruses
Selectable marker genes and reporter genes
Resistance to African cassava mosaic virus
Common use(s)
Food
Additional Information
Additional Information
RNA interference
An RNAi response is an anti-viral response triggered by the recognition of dsRNA. Host DICER recognizes dsRNA, cleaving the dsRNA into small interfering RNA (siRNA), roughly 21-23 bp long (size is host dependent). The siRNA is then bound by ARGONAUTE family proteins, which unwind the duplex, leaving a single strand of the siRNA, and activating the RISC complex. The RISC complex targets transcripts with homology to the siRNA and degrades them.
Other relevant website address or attached documents
GenBank: African cassava mosaic virus-[Nigeria-Ogo] AC1 gene, AC2 gene, AC3 gene, AC4 gene, AV2 gene and AV1 gene
Records referencing this document
(
4
)
ID
Description
4
record(s) found
Modified Organism
4 records
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