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Modified Organism
CTC-91Ø87-6 - Insect resistant sugarcane
Record information and status
Record ID
115593
Status
Published
Date of creation
2020-05-29 20:01 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-05-29 20:01 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant sugarcane
Transformation event
CTC91087
Unique identifier
CTC-91Ø87-6
Developer(s)
Dr Wladecir Salles Oliveira
Chairman of the Internal Biosafety Committee
CTC – Centro de Tecnologia Canavieira (CTC)
Fazenda Santo Antônio s/n, Caixa Postal 162
Piracicaba, Sao Paulo
Brazil, 13400-970
Phone:+55 (11) 3429-8111
Fax:+55 (11) 3429-8111
Email:Wladecir.oliveira@ctc.com.br
Url:CTC
Description
The sugarcane was modified for resistance to sugarcane borer (Diatraea saccharalis) through the expression of Bacillus thuringiensis Cry1Ac. The sugarcane additionally contains Streptomyces hygroscopicus phosphinothricin N-acetyltransferase gene as a selectable marker.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Saccharum officinarum L. - Sugarcane, Sugar cane
Point of collection or acquisition of the recipient organism
Sugarcane early variety CTC9001
Characteristics of the transformation process
Vector
p91087
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ubiquitin gene promoter
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Cry1Ac
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Nopaline Synthase Gene Terminator
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Ubiquitin gene promoter
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Phosphinothricin N-acetyltransferase gene
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Genetic cassettes:
The modified sugarcane contains two gene cassettes: Bacillus thuringiensis cry1Ac and Streptomyces hygroscopicus phosphothricin N-acetyltransferase (pat). Both gene cassettes are under transcriptional control of the Zea mays ubiquitin promoter and the Agrobacterium tumefaciens nopaline synthase terminator.

Notes:
- The coding sequence for cry1Ac was optimized for expression in sugarcane (sugarcane preferred codons).
- High levels of transcription are expected due to the constitutive nature of the ubiquitin promoter.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
  • Biofuel
Detection method(s)
Additional information
The Cry1Ac encodes a 615 amino acid protein with an estimated molecular weight of 68 kDa. The insecticidal core of the protein is 52 kDA.

DNA and protein on sugar samples produced from this modified sugarcane were unable to detection Cry1Ac or PAT (below the levels of detection) according Gianotto et al. (2019) (see attached document).
Additional Information
Other relevant website address or attached documents