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Modified Organism
TIC-AR233-5 - Potato Virus Y-resistant Potato
Record information and status
Record ID
115053
Status
Published
Date of creation
2019-07-17 19:28 UTC (austein.mcloughlin@cbd.int)
Date of last update
2019-07-17 19:30 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-07-17 19:30 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato Virus Y-resistant Potato
Transformation event
SY233
Unique identifier
TIC-AR233-5
Developer(s)
Tecnoplant SA
Tecnoplant SA
Constitución 4234
(1254) Capital Federal
Buenos Aires, Capital Federal
Argentina
Phone:+54 11 4909 8138
Fax:+54 11 4909 8055
Url:Tecnoplant (en Español)
Description
The potato was modified for resistance to Potato Virus Y infection. The modified potato expresses the viral coat protein to cross-protect the plant from viral inoculation, spread, multiplication and symptom expression. Although the mechanism is not well-defined, likely the production of the coat protein enhances the production of movement proteins and coordinates the formation of viral replication complexes, thus limiting the spread of the viral via cell-to-cell infections. Additionally, the modified potato expresses neomycin phosphotransferase II for kanamycin selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
pBI121
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Nopaline Synthase Gene Promoter
0.18 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.35 Kb
 
 
PVY coat protein
1.27 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The modified potato (Solanum tuberosum) contains two genetic cassettes for expression of  Escherichia coli neomycin phosphotransferase II (nptII) and Potato Virus Y (PVY) coat protein.

Transcription of nptII begins at an Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminates at an A. tumefaciens nos terminator. Neomycin phosphotransferase is a selectible marker used for selecting transformants.

Transcription of PVY coat protein initiates from a Cauliflower Mosaic Virus 35S promoter and terminates at a A. tumefaciens nos terminator. The sequence of the PVY coat protein contains the last 17 codons of the viral replicase gene, the full coat protein sequence and the 3' noncoding region.

Note:
- Southern blot analysis indicated multiple transgene insertions.
LMO characteristics
Modified traits
Common use(s)
  • Food
Detection method(s)
Additional information
Western blot analysis indicated that NPTII was present and readily detected from leaf and tuber samples. From leaf extracts, the PVY coat protein was difficult to detect.

PCR analysis confirmed the presence of both nptII and PVY coat protein.

Southern blot analysis indicated three insertions of the PVY coat protein.

Northern blot analysis detected low levels of PVY coat protein transcript in root, stem and leaf tissues. Detection relied on extended autoradiographic exposure.

For more information, kindly refer to the attached article (Bravo-Almonacid et al., 2012)*.

*Note: Methods used in the article are not validated.