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Modified Organism
IR-ØØGR2E-5 - Provitamin A biofortified rice
Record information and status
Record ID
112997
Status
Published
Date of creation
2018-01-10 17:58 UTC (dina.abdelhakim@cbd.int)
Date of publication
2018-01-10 17:58 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Provitamin A biofortified rice
Transformation event
GR2E
Unique identifier
IR-ØØGR2E-5
Developer(s)
International Rice Research Institute
Laguna
Philippines
Description
Rice event GR2E (IR-ØØGR2E-5) was developed through the use of recombinant-DNA techniques to express elevated levels of provitamin A (mainly β-carotene) in the rice endosperm, which is converted in the body to vitamin A.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Oryza sativa - Rice, ORYSA
Point of collection or acquisition of the recipient organism
japonica rice cultivar Kaybonnet
Characteristics of the transformation process
Vector
pSYN12424
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ubiquitin gene promoter
1.99 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Glutelin gene promoter
0.83 Kb
 
 
Phytoene Synthase 1 gene
1.23 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Glutelin gene promoter
0.83 Kb
 
 
rbcS Transit Peptide
0.17 Kb
 
 
Phytoene Desaturase gene
1.48 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Based on molecular characterization of GR2E rice, one copy of the pSYN12424 T-DNA was introduced at a single site within the rice genome and stably inherited over multiple generations as a single genetic locus according to Mendelian rules of inheritance. In addition, nucleotide sequencing of the entire inserted DNA, including portions of the 5' and 3' flanking rice genomic sequence, confirmed that the T-DNA had been inserted without modifications, deletions, or rearrangements, except for small truncations at the 5' and 3' termini of 23 bp and 11 bp, respectively. There were also no new novel open reading frames created as a consequence of the DNA insertion that would have the potential to encode a protein with any significant amino acid sequence similarity to known and putative toxins or allergens.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record