In plant cells a glucose analog, 2-deoxyglucose (2-DOG), is
phosphorylated by hexokinase to form 2-DOG-6-phosphate, which
competes with glucose-6-phosphate causing cell death through the
inhibition of glycolysis, protein synthesis, cell wall
polysaccharide synthesis and also protein glycosylation.
DOGR1 gene, which has been isolated from Saccharomyces cerevisiae
strain S288C, encodes 2-deoxyglucose-6-phosphate. The enzyme has
the ability to convert toxic 2-DOG-6-phosphate to non-toxic
products through dephosphorylation.
Therefore, transformed plant cells which carry the DOGR1 gene can
be selected on medium containing 2-DOG.
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