Two fragments of 170 and 191 bp, corresponding to the most
conserved regions from, respectively, α- and ω-gliadins, were
selected for use as interfering RNA (IR) fragments. These fragments
were amplified from bread wheat, assembled to obtain the full
chimeric 361-bp ω/α fragment, cloned into the recombination sites
of pCR8/GW/TOPO vector (Invitrogen), and recombined to provide the
final pDhp-ω/α vector.
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