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Sugar beet modified for herbicide tolerance
Monsanto Europe S.A.
Avenue de Tervuren 270-272
The T227-1 sugar beet (Beta vulgaris) was modified with
the insertion of Agrobacterium tumefaciens
5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) to confer
herbicide tolerance to the glyphosate. The variation in the
bacterial enzyme allows the plant to tolerate otherwise lethal
doses of glyphosate.
The beet lines T227-1 and H7 (Record ID: 14773 - see below) are
independent transformation lines containing the same genetic
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
- Agrobacterium-mediated DNA transfer
Chloroplast transit peptide 2
5-enolpyruvylshikimate-3-phosphate synthase gene
rbcS-E9 gene terminator
Transcription of the Agrobacterium tumefaciens
5-enolpyruvylshikimate-3-phosphate synthase (epsps) gene is under
control of the Figwort Mosaic Virus 35S promoter and the Pisum
sativum Rubisco small subunit (rbcS-E9) terminator. The
transcript contains an Arabidopsis thaliana chloroplast
transit peptide at the 5' end of the transcript to facilitate
post-translational targeting to the chloroplast. Due to the viral
promoter, transcription is expected to occur constitutively at
Please note: the nucleic acid sequence of the epsps gene was
codon-optimized for expression in plants.
The sugar beet lines T227-1 and H7-1 are independent transformation
events harbouring the same genetic elements.