|
|
english
|
español
|
français
|
Sign Up for an Account
|
Sign In
Country Profiles...
Home
The BCH
The Protocol
Finding Information
Registering Information
Resources
Help
Finding Information
National Contacts
Laws and Regulations
Country's Decisions and other Communications
Submissions
Risk Assessments
Roster of Experts
LMOs, Genes or Organisms
National Reports
Capacity-Building
Organizations
The BCH Virtual Library
Survey on indicators (2014)
Compiled Information
National Contacts
LMO Registry
Organism Registry
Gene Registry
Go to record ID
Home
|
Finding Information
|
Record details
Modified Organism
Barley modified for tolerance to drought and nutrient deficiencies
LMO Information
Decisions on the LMO
Risk Assessments
Record information and status
Record ID
108704
Status
Published
Date of creation
2015-09-02 19:17 UTC (hana.jirakova@gmail.com)
Date of last update
2015-09-10 22:15 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-09-10 22:15 UTC (dina.abdelhakim@cbd.int)
Tweeter
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the
LMO quick-links
page.
LMO name
Barley modified for tolerance to drought and nutrient deficiencies
Transformation event
bGLU:CKX1
Developer(s)
Record #108702
Palacky University in Olomouc
Faculty of Science
Krizkovskeho 8
Olomouc
Czech Republic, 771 47
Phone:
+420 585 634 060
Fax:
+420 585 634 002
Email:
dekanat.prf@upol.cz
Url:
Facuty of Science, the Palacky University in Olomouc
Description
The bGLU:CKX1 barley line has been genetically modified by inserting the Arabidopsis thaliana cytokinin dehydrogenase 1 gene under the control of the root-specific β-glucosidase promoter from maize.
Cytokinin dehydrogenases catalyze the irreversible degradation of cytokinins in a single enzymatic step by oxidative side chain cleavage. Cytokinins, which are chemically N6-substituted purine derivatives, are a class of plant hormones that regulate cell division as well as a large number of developmental events in plants. An important trait regulated by cytokinin is the size of the root system.
A reduced cytokinin status in plants (including barley) causes an enhanced root system which might render plants more tolerant to drought and nutrient deficiencies in the soil. Crop yield is often limited by the availability of water and soil-derived mineral nutrients. A larger root system may enable plants to get better access to water and nutrient resources, therefore to better cope with adverse environmental conditions.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Record #12110
Hordeum vulgare - Barley, HORVU
Point of collection or acquisition of the recipient organism
cv. Golden Promise
Related LMOs
Record #108706
Barley modified for tolerance to drought and nutrient deficiencies
Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes Tolerance to abiotic stress - Drought
Record #108708
Barley modified for tolerance to drought and nutrient deficiencies
Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes Tolerance to abiotic stress - Drought
Record #108710
Barley modified for tolerance to drought and nutrient deficiencies
Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes Tolerance to abiotic stress - Drought
Characteristics of the transformation process
Vector
bGLU:CKX1
Techniques used for the modification
Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Record #108700
Beta-glucosidase gene promoter - Zea mays - Maize, Corn, MAIZE
Record #108701
Cytokinin dehydrogenase 1 gene - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
Tolerance to abiotic stress - Drought
Record #108703
TMV 35s promoter - Tobacco mosaic virus - TMV
Record #14991
Hygromycin B phosphotransferase gene - Escherichia coli - ECOLX
Resistance to antibiotics - Hygromycin
Selectable marker genes and reporter genes
Notes regarding the genetic elements introduced or modified in this LMO
The transgenic line shows an up to 8-fold higher CKX enzyme activity in roots as compared to the wild type. In contrast, CKX enzymatic activity in the leaves of transgenic plants is similar to the control plants.
The cytokinin dehydrogenase 1 gene is under the control of the root-specific β-glucosidase promoter from maize.
The Hygromycin B phosphotransferase selection marker is under the control of the TMV 35s promoter.
LMO characteristics
Modified traits
Resistance to antibiotics
Hygromycin
Selectable marker genes and reporter genes
Tolerance to abiotic stress
Drought
Common use(s)
Research
Records referencing this document
(
4
)
ID
Description
4
record(s) found
Modified Organism
3 records
Risk Assessment
1 record
Contact us
|
Site Map
|
Linkages Policy
|
Privacy Policy
|
Disclaimer
|
© SCBD 2001-2016
Rate this page
- 65 people have rated this page