Record details

Living Modified Organism identity

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LMO name

Maize modified for insect resistance and herbicide tolerance

Transformation event

98140 x TC1507

Unique identifier

DP-Ø9814Ø-6 x DAS-Ø15Ø7-1

Developer(s)

Record #14931

Pioneer Hi-Bred International Inc.

7100 NW 62nd Avenue
PO Box 1000

Johnston, Iowa

United States of America, 50131

Phone:	+1 515 535-3200
Url:	Pioneer HiBred International Homepage

Description

The stacked maize line DP-Ø9814Ø-6 and DAS-Ø15Ø7-1 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of the cry1F, gat4621, phosphinothricin acetyltransferase, and acetohydroxyacid synthase genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate, Sulfonylurea and glyphosate herbicide.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #246

Zea mays - Maize, Corn, MAIZE

Record #48466

DP-Ø9814Ø-6 - Optimum™ GAT™ maize

Resistance to herbicides - Glyphosate, Sulfonylurea

Show detection method(s)

Record #14841

DAS-Ø15Ø7-1 - Herculex™ I maize

Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate

Show detection method(s)

Characteristics of the transformation process

Vector

PHP24279 and PHI8999A

Techniques used for the modification

Cross breeding

Genetic elements construct

CaMV 35S promoter

#100287

0.55 Kb

Phosphinothricin N-acetyltransferase gene

#15002

0.55 Kb

CaMV 35S terminator

#100290

0.20 Kb

Ubiquitin gene promoter

#100362

1.98 Kb

Cry1F

#14987

1.82 Kb

ORF25 PolyA Terminator sequence

#100363

0.72 Kb

Ubiquitin gene promoter

#100362

0.90 Kb

Ubiquitin 5' Untranslated Region

#103927

0.08 Kb

Ubiquitin Intron 1

#103627

1.01 Kb

Glyphosate-N-Acteyltransferase gene

#48363

0.44 Kb

Proteinase inhibitor II gene terminator

#100367

0.32 Kb

Proteinase inhibitor II gene terminator

#100367

0.31 Kb

Acetohydroxyacid synthase gene

#48364

1.92 Kb

Acetohydroxyacid synthase gene promoter

#103926

0.66 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

DNA insert from DP-Ø9814Ø-6 vector PHP24279
Expresses the GAT4621 and ZM-HRA proteins. The GAT4621 protein is a glyphosate acetyltransferase (GAT), confers tolerance to the herbicide glyphosate. The acetohydroxyacid synthase (AKA acetolactate synthase) gene confers tolerance to ALS-inhibiting herbicides, such as chlorimuron and thifensulfuron.

DNA insert from DAS-Ø15Ø7-1 vector PHI8999A
Expresses the Cry1F gene from Bacillus thuringiensis to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.

For additional information on this LMO, please refer to the records of the parental LMOs.

LMO characteristics

Modified traits

Resistance to diseases and pests
- Insects
  - Lepidoptera (butterflies and moths)
Resistance to herbicides
- Glufosinate
- Glyphosate
- Sulfonylurea

Common use(s)

Food
Feed

Detection method(s)

External link(s)

DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

Additional Information

Other relevant website address or attached documents

DP-9814Ø-6xDAS-Ø15Ø7-1 - OECD