Record details

Living Modified Organism identity

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LMO name

Plum Tree modified for resistance to Plum pox virus

Transformation event

Unique identifier

ARS-PLMC5-6

Developer(s)

Record #104297

Dr. Ralf Scorza

Research Horticulturist and Lead Scientist

Agricultural Research Service

United States Department of Agriculture

2217 WILTSHIRE ROAD Kearneysville, WV 25430

Kearneysville, West Virginia

United States of America, 25430

Phone:	304 725 3451 322
Email:	Ralph.Scorza@ars.usda.gov

Description

Plum tree Stanley clone C5 was modified to contain the coat protein gene of Plum pox virus. The insertion of the construct changes the properties of the recipient's organism so that plum obtains Plum pox virus coat protein gene into its genetic information. This gene encodes the synthesis of a pox virus coat protein which in turn confers resistance against the PPV virus.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #104307

Prunus domestica - Plum tree, Plum

Point of collection or acquisition of the recipient organism

Cultivar: Stanley; Clone: C5

Characteristics of the transformation process

Vector

pGA482GG/PPVCP-33

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

Nopaline Synthase Gene Promoter

#100270

0.25 Kb

Neomycin Phosphotransferase II

#15001

0.79 Kb

Nopaline Synthase Gene Terminator

#100269

1.20 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

PPV coat protein

#104309

1.25 Kb

CaMV 35S promoter

#100287

0.45 Kb

CaMV 35S promoter

#100287

0.85 Kb

Beta-Glucuronidase coding sequence

#46004

1.81 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

Clone C5 appeared to have at least two inserts with one being a possible rearrangement of the PPV-CP gene. This plant had significantly lower amounts of RNA transcript and no detectable protein.

LMO characteristics

Modified traits

Resistance to antibiotics
- Kanamycin
Resistance to diseases and pests
- Viruses

Common use(s)

Food
Research

Detection method(s)

External link(s)

ARS-PLMC5-6 - GMO Detection method Database (GMDD)

Additional Information

Other relevant website address or attached documents

Transgenic plums (Prunus domestica L.) express the plum pox virus coat protein gene.pdf

C5 Plum resistant to Plumpox Virus - APHIS

ARS-PLMC5-6 (C5) - CERA